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目的研究50Hz磁场对细胞缝隙连接通讯(GJIC)功能抑制作用的剂量效应关系及其有关机理。方法利用微注射法将荧光染料引入单个中国仓鼠肺来源成纤维(CHL)细胞内,以5分钟后的荧光偶合细胞数作为GJIC功能的指标。研究了不同磁场强度、不同辐照时间对CHL细胞GJIC的影响。结果工频磁场对GJIC的抑制作用与其强度有关,低强度(005、02和04mT)的磁场辐照24小时不能抑制GJIC,高强度(08和16mT)的磁场辐照24小时能够抑制GJIC。在0~16mT范围内,磁场对GJIC的抑制作用不呈“强度窗”效应。将强度固定为08mT,5分钟的辐照无作用,1小时的辐照足以抑制GJIC,24小时的辐照对GJIC的抑制作用最明显。此外,研究发现对GJIC的抑制主要是工频磁场本身的直接作用,而并非由磁场在被辐照物中受感应的电场所致。结论工频磁场对GJIC的抑制具有剂量效应关系。
Objective To study the doseeffect relationship and its related mechanism of 50 Hz magnetic field on GJIC inhibition. Methods Fluorescent dyes were introduced into a single Chinese hamster lung-derived fibroblast (CHL) cells by microinjection, and the number of fluorescently-coupled cells after 5 minutes was used as an index of GJIC function. The effect of different magnetic field intensity and different irradiation time on the GJIC of CHL cells was studied. Results The magnetic field of GJIC was attenuated by the applied magnetic field. The low intensity (005, 02, and 04 mT) magnetic field irradiation for 24 hours did not inhibit GJIC, high intensity (08 and 16 mT) Magnetic field irradiation for 24 hours inhibited GJIC. In the range of 0 ~ 1 6mT, the inhibition effect of magnetic field on GJIC does not show the “strength window” effect. The intensity was fixed at 0 8mT, 5 minutes of irradiation had no effect, 1 hour irradiation was sufficient to inhibit GJIC, 24 hours of irradiation on the inhibition of GJIC most obvious. In addition, the study found that the inhibition of GJIC is mainly the direct effect of power frequency magnetic field itself, not caused by the electric field induced by the magnetic field in the irradiated object. Conclusion The frequency-magnetic field has dose-effect relationship to the inhibition of GJIC.