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目的:比较梅毒酶联免疫吸附试验(ELISA)、梅毒快速血浆反应素检测(RPR)、甲苯胺红不加热血清试验(Trust)、梅毒螺旋体明胶凝聚试验(TPPA)四种方法检测梅毒螺旋体抗体的结果,选择最有效的梅毒检测方法。方法:以270例确诊的梅毒患者为研究组,270例未感染梅毒的正常人群为对照组。所有入选对象采用真空采血管取静脉血5ml,并进行ELISA、RPR、Trust、TPPA检测。记录研究组和对照组阳性数或假阳性数,并计算两组阳性率或假阳性率。比较不同检查方法下阳性率或假阳性率是否存在统计学差异。结果:ELISA和TPPA阳性率分别为95.19%、95.56%,均显著高于RPR和TRUST(p<0.05)。ELISA和TPPA假阳性率分别为1.11%、1.48%,均显著低于RPR和TRUST(p<0.05)。结论:相比于RPR和Trust,ELISA和TPPA在梅毒检测中具有更好的运用价值,值得在临床推广运用。
OBJECTIVE: To compare the detection of syphilis antibody against syphilis by ELISA, syphilis rapid plasma assay (RPR), toluidine red unheated serum (TRP) and Treponema pallidum gelatin agglutination test (TPPA) As a result, the most effective method of syphilis detection is selected. Methods: 270 cases of confirmed syphilis patients as study group, 270 cases of normal people without syphilis as control group. All subjects were taken by vacuum blood collection venous blood 5ml, and ELISA, RPR, Trust, TPPA detection. Record the positive number or false positive number of the study group and the control group, and calculate the positive rate or false positive rate of the two groups. To compare the positive rate or false positive rate of different methods there is a statistical difference. Results: The positive rates of ELISA and TPPA were 95.19% and 95.56%, respectively, which were significantly higher than those of RPR and TRUST (p <0.05). The false positive rates of ELISA and TPPA were 1.11% and 1.48% respectively, which were significantly lower than those of RPR and TRUST (p <0.05). CONCLUSIONS: Compared with RPR and Trust, ELISA and TPPA have better value in the detection of syphilis and are worthy of clinical application.