目前,胆碱酯酶(ChE)活性的测定方法颇多,其中用的最多的是Ellman(1961)测定法,该方法具有灵敏度高、简便及有一定的专一性等优点(张壬午等,1983),但是,硫代胆碱和DTNB的反应受介质pH值的影响,因此,Ellman测定程序被限制在一定的pH值范围内(DTNB二硫键的断裂要求pH值在6.5—8.5之间)。另一方面,由于蛋白质游离-SH基 At present, there are many methods for determination of cholinesterase (ChE) activity, among which Ellman (1961) is the most used method. The method has the advantages of high sensitivity, simpleness and certain specificity (Zhang et al., 1983 ), However, the reaction of thiochol with DTNB is affected by the pH of the medium and therefore the Ellman assay procedure is limited to a range of pH values ​​(breakage of DTNB disulfide bonds requires pH values ​​between 6.5 and 8.5) . On the other hand, due to the protein free-SH group