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目的采用DPPH·法,测定云厚朴SFE-CO2萃取液、乙醇提取液和大紫丹参水提取液清除DPPH·自由基的活性。方法通过在样品中加入DPPH·,测定吸光度的改变,计算清除率%和C50。结果云厚朴SFE-CO2萃取液C50=4.83μg/mL,云厚朴乙醇提取液C50=4.17μg/mL;尚未达到C50时,大紫丹参水提取液的清除曲线就趋于平缓。结论云厚朴SFE-CO2萃取液、乙醇提取液和大紫丹参水提取液都具有清除DPPH·自由基的活性,大紫丹参水提取液清除DPPH·自由基的作用较弱。
OBJECTIVE To determine DPPH · free radical scavenging activity of DPPH · SF, extract of SFE-CO2, ethanol extract and water extract of Radix Salviae miltiorrhizae. Methods The change in absorbance was measured by adding DPPH · to the sample and the% clearance and C50 were calculated. Results C50 = 4.83μg / mL of SFE-CO2 extract of Cortex Magnoliae Officinalis, and C50 = 4.17μg / mL of CTP of Cortex Magnoliae Officinalis. When the C50 was not reached yet, the scavenging curve of aqueous extract of Radix Salviae Miltiorrhizae tended to be gentle. Conclusions The extracts from SFE-CO2, ethanol extract and Salvia miltiorrhiza Bunge have activity of scavenging DPPH · free radical. The scavenging effect of aqueous extract of Radix Salviae Miltiorrhizae on DPPH · Radical is weak.