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了解云南省2009~2014年甲型H1N1流感病毒的流行趋势,研究HA和NA基因进化特征。对云南省近6年来上报的流感监测病例数据进行病原谱总结,挑选出23株甲型H1N1流感毒株进行HA及NA基因分析。利用MEGA 5.0软件对测序结果构建进化树分析基因同源性。2009~2014年云南省共监测到4次甲型H1N1流感流行高峰,核酸检测结果中甲型H1N1流感占检出总量的28.8%。测序结果显示,HA与NA基因均分为3个类群,检测到一株具有H275Y突变位点的毒株。甲型H1N1流感是导致本省流感流行的重要亚型之一,2009~2014年间分离的毒株主要有Goup1、Gourp7和Gourp6三个支系,绝大部分甲型H1N1流感毒株仍对神经氨酸酶抑制剂敏感。
To understand the epidemic trend of influenza A (H1N1) virus in Yunnan Province from 2009 to 2014 and to study the evolutionary characteristics of HA and NA genes. A total of 23 influenza A (H1N1) strains were selected for HA and NA gene analysis of the influenza surveillance cases reported in Yunnan Province in recent 6 years. Phylogenetic tree analysis of gene sequences using MEGA 5.0 software. From 2009 to 2014, a total of 4 influenza A (H1N1) epidemic peaks were detected in Yunnan Province, accounting for 28.8% of the total detected in the nucleic acid test. The sequencing results showed that the HA and NA genes were divided into three groups and a strain with the H275Y mutation was detected. Influenza A (H1N1) is one of the important subtypes that has led to the pandemic influenza in the province. The strains isolated from 2009 to 2014 mainly include three strains of Goup1, Gourp7 and Gourp6. Most of the strains of the type A H1N1 still exert a negative effect on neuraminidase Enzyme inhibitor sensitive.