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目的 :用水提取的白花蛇舌草多糖常含有蛋白质 ,研究除蛋白方法。方法 :用Sevag法和TCA法分别除蛋白 ,分别测定所得样品的总糖和蛋白质含量 ,比较两个方法的优劣。结果 :Sevag法除蛋白 ,复杂、费时 ,通常需要数十次 ,样品损失很大 ,用TCA方法除蛋白则无须重复许多次 ,节省时间 ,所需试剂也大量节省 ,多糖损失减少 ,还能除去大量色素。结论 :用TCA方法除蛋白效果较好。
Purpose : The extracted Hedyotis diffusa polysaccharides often contain proteins to study the deproteinization method. Methods: The Sevag method and TCA method were used to remove proteins, and the total sugar and protein contents of the samples were determined respectively. The advantages and disadvantages of the two methods were compared. Results: The Sevag method is complex, time-consuming and usually requires dozens of times. The sample loss is very large. The protein removal by TCA method does not have to be repeated many times, saves time, requires a lot of reagents, reduces polysaccharide loss, and can also remove A lot of pigments. Conclusion: The effect of removing protein by TCA method is better.