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目的研究不同剂量光动力作用后C6胶质瘤细胞内游离钙犤Ca2+犦i的变化,探讨钙超载在光动力诱导C6胶质瘤细胞坏死和凋亡过程中的作用。方法常规培养C6胶质瘤细胞,以不同光动力剂量处理对数生长期的实验组细胞后,应用Fluo-3/AM为细胞内钙离子荧光指示剂,激光共聚焦显微镜测定各组C6胶质瘤细胞内游离钙的变化;MTT法检测各组细胞的死亡率。结果光动力作用后C6胶质瘤细胞内游离钙明显升高,平均胞内钙离子浓度和细胞死亡率随着光动力剂量增加而增大。结论光动力作用后C6细胞钙超载在光动力诱导肿瘤细胞坏死和凋亡过程中起重要作用。
Objective To study the changes of intracellular calcium (Ca2 +) i in C6 glioma cells under different doses of photodynamic therapy, and to explore the role of calcium overload in the photodynamic therapy of C6 glioma cells. Methods C6 glioma cells were cultured routinely and treated with different doses of photodynamic therapy in logarithmic growth phase group. Fluo-3 / AM was used as intracellular calcium ion fluorescent indicator. The contents of C6 glial were measured by confocal microscopy The changes of intracellular free calcium in the tumor cells. The cell death rates in all groups were detected by MTT assay. Results The intracellular free calcium in C6 glioma cells was significantly increased after photodynamic therapy. The mean intracellular calcium concentration and cell death rate increased with the increase of photodynamic dose. Conclusion The calcium overload of C6 cells plays an important role in photodynamic therapy of tumor necrosis and apoptosis.