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1950年Fricdman氏首先用硫酸小蘖硷等染色,在熒光显微镜下观察了阴道涂片中的脱落癌细胞。同年Schummelfeder氏就使用了(口了)叮橙做为熒光染料。从此以后在医学文献中,出现不少用吖叮橙染色,以熒光显微镜检查癌细胞的资料。到1958年,Ludwig von Bertalanffy氏用于诊断宫颈癌,以后F.D.Bertalanffy氏和Grubb氏分别应用此法检查了胸腹水,以及胃液和痰中的脱落细胞;就较多的资料,他们分析了用熒光显微旬检查癌细胞的准确率,一般认为高于巴
In 1950, Fricdman’s was first stained with barium sulfate, etc., and the exfoliated cancer cells in the vaginal smear were observed under a fluorescence microscope. In the same year, Schummelfeder’s used an orange as a fluorescent dye. Since then, in the medical literature, there have been many cases of staining cancer cells with fluorescent microscopy. By 1958, Ludwig von Bertalanffy’s was used for the diagnosis of cervical cancer. Later, FDBertallanffy’s and Grubb’s used this method to examine pleural effusion and ascites, as well as exfoliated cells in gastric juice and sputum; for more information, they analyzed the use of fluorescence. Microscopic examination of the accuracy of cancer cells is generally considered higher than