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目的探讨c-Jun/激活蛋白1(AP-1)在活化的凝血因子Ⅶa促进SW620细胞增殖、迁移过程中的作用及其调控机制。方法 Western blot检测人结肠癌SW620细胞中Ⅶa、组织因子(TF)、蛋白酶激活受体2(PAR2)、细胞外调节蛋白激酶1/2(ERK1/2)抑制剂U0126、p38抑制剂SB203580处理后c-Jun、磷酸化c-Jun(p-c-Jun)的蛋白水平变化;MTT和Transwell法分别检测AP-1抑制剂姜黄素对细胞增殖和细胞迁移能力的影响。结果单克隆抗TF和PAR2抗体、U0126均能抑制Ⅶa促进SW620细胞中c-Jun/AP-1活化的过程(P<0.05)。姜黄素降低Ⅶa诱导的SW620细胞增殖和细胞迁移能力(P<0.05)。结论 TF/Ⅶa复合物通过激活PAR2促进SW620细胞增殖和迁移,c-Jun/AP-1在此过程中被激活并发挥重要作用,ERK1/2为c-Jun/AP-1上游分子具有调控效应。
Objective To investigate the role of c-Jun / Activin-1 (AP-1) in the proliferation and migration of SW620 cells induced by activated factor Ⅶ a and its regulatory mechanism. Methods The expressions of Ⅶ a, tissue factor (TF), activator of proteinase 2 (PAR2), inhibitor of extracellular regulated protein kinase 1/2 (ERK1 / 2) U0126 and inhibitor of p38 SB203580 in human colon cancer SW620 cells were detected by Western blot c-Jun, and phosphorylated c-Jun (pc-Jun). The effects of AP-1 inhibitor curcumin on cell proliferation and cell migration were detected by MTT and Transwell assay. Results Monoclonal anti-TF and PAR2 antibodies, U0126, both inhibited the activation of c-Jun / AP-1 in SW620 cells by Ⅶ a (P <0.05). Curcumin reduced the proliferation and cell migration ability of SW620 cells induced by Ⅶ a (P <0.05). Conclusions TF / Ⅶ a complex can promote the proliferation and migration of SW620 cells by activating PAR2. C-Jun / AP-1 is activated and plays an important role in this process. ERK1 / 2 has regulatory effect on upstream molecules of c-Jun / AP- .