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目的:探讨解毒化瘀药含药血清对白血病K562/A02耐药细胞凋亡的影响。方法:应用MTT法检测解毒化瘀药含药血清对K562/A02耐药细胞的生长抑制率,流式细胞仪检测解毒化瘀药含药血清处理后的各组K562/A02细胞凋亡的情况。结果:MTT细胞毒结果显示解毒化瘀药血清能够抑制K562/A02的生长,其抑制率随药物浓度增加而增加,流式细胞检测结果显示解毒化瘀药能促进白血病耐药细胞K562/A02凋亡率,并呈浓度相关性。结论:解毒化瘀药含药血清能够促进K562/A02细胞凋亡。
Objective: To investigate the effect of drug-containing serum of Jiedu Huayu drug on the apoptosis of K562 / A02 drug resistant leukemia cells. Methods: MTT assay was used to detect the growth inhibition rates of K562 / A02 drug-resistant serums, and the apoptosis of K562 / A02 cells was detected by flow cytometry . Results: The results of MTT cytotoxicity showed that Jiedu Huayu serum could inhibit the growth of K562 / A02 cells. The inhibition rate increased with the increase of drug concentration. The results of flow cytometry showed that Jiedu Huayu drug could promote the proliferation of K562 / A02 cells Mortality, and showed a concentration-related. Conclusion: The drug-containing serum of Jiedu Huayu can promote the apoptosis of K562 / A02 cells.