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目的动物实验得知睾丸间质细胞激素胰岛素样因子3(insulin-like factor 3,Ins13)在腹部睾丸迁移过程中起到非常重要的作用。此过程在人类发生在妊娠中期(第二个3个月),本研究探讨是否人体中Ins13在睾丸下降过程中也起到一定作用。方法经羊膜穿刺术60名孕妇取羊水测定Ins13及睾酮(testosterone,T)水平,选取样本孕周15-25w,确定胎儿男性28例,女性32例。结果 28例孕男性胎儿羊水中均检测到Ins13(160.5±85.2pg/ml),而在孕女性胎儿羊水中均未检测到此激素水平。T水平在男性胎儿(1.05±0.30 nmol/L)与女性胎儿(0.33±0.05 nmol/L)相比具有显著差异性(P<0.0001)。在男性胎儿中Ins13与T阴性相关,Ins13与孕龄统计学阴性相关处于基线水平(P=0.06),T与孕龄不相关(P=0.10);相反女性胎儿T水平与孕龄相关(P=0.03)。结论 Ins13存在于人类孕中期孕有男性胎儿羊水中,而孕中期正值睾丸移位过程中。相反在女性胎儿羊水中未检测到Ins13,结果认为Ins13在人类睾丸下降过程同样起到重要作用。
Objective Animal experiments show that testicular stromal cell hormone insulin-like factor 3 (Ins13) plays a very important role in the process of abdominal testicular migration. This process occurs in humans during the second trimester of pregnancy (the second trimester) and this study explores whether Ins13 also plays a role in the testicular decline in humans. Methods Amniocentesis was performed by amniocentesis in 60 pregnant women to determine the levels of Ins13 and testosterone (T). Samples were selected for gestational weeks 15-25w, and 28 males and 32 females were determined. Results Ins13 (160.5 ± 85.2 pg / ml) was detected in 28 pregnant women with fetal amniotic fluid, while no such hormone was detected in pregnant women with fetal amniotic fluid. T levels were significantly different (P <0.0001) in male fetuses (1.05 ± 0.30 nmol / L) compared to female fetuses (0.33 ± 0.05 nmol / L). Ins13 was negatively correlated with T in male fetuses, while Ins13 was negatively correlated with gestational age at baseline (P = 0.06). T was not associated with gestational age (P = 0.10); on the contrary, female fetus T was associated with gestational age (P = 0.03). Conclusion Ins13 is present in human fetal amniotic fluid in the second trimester of pregnancy, and is positive in the second trimester of pregnancy. In contrast, Ins13 was not detected in the fetal fluid of female fetuses, suggesting that Ins13 also plays an important role in the decline of human testes.