Narrow disperse poly(ethyleneglycol dimethacrylate-co-4-vinylpyridine) (poly(EGDMA-co-4-VPy)) microspheres were prepared by distillation-precipitation copolymerization of ethyleneglycol dimethacrylate (EGDMA) and 4-vinylpyridine (4-VPy) with 2,2-azobisisobutyronitrile (AIBN) as initiator in neat acetonitrile. The polymer microspheres containing pyridyl group were then utilized as stabilizer for gold metallic colloids with the diameter around 7 nm, which were prepared by the in situ reduction of gold chloride trihydrate with sodium borohydride through the coordination of the pyridyl group on the gel layer and surface of the microsphere with the gold metallic nano-particles. The catalytic properties of the pyridyl-functionalized microsphere-stabilized gold metallic colloids and the behavior of the stabilized-catalyst for the recycling were investigated with reduction of 4-nitrophenol to 4-aminophenol as a model reaction.