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The effects of Tween-20 and Zn(Ⅱ) on ethylbenzene removal were evaluated using two biotrickling filters (BTFs),BTF1 and BTF2.Only BTF1 was fed with Tween-20 and Zn(Ⅱ).Results show that ethylbenzene removal decreased from 94% to 69% for BTF1 and from 74%to 54% for BTF2 with increased organic loading from 64.8 to 189.0 g ethylbenzene/(m3·hr) at EBRT of 40 sec.The effect of EBRT (60-15 sec) at a constant ethylbenzene inlet concentration was more significant than that of EBRT (30-10 sec) at a constant organic loading.Biomass accumulation rate within packing media was reduced significantly.