论文部分内容阅读
目的:建立RP-HPLC法同时测定癌痛平中重楼皂苷Ⅰ、Ⅱ、Ⅵ和Ⅶ的含量测定方法。方法:采用Agilent ZORBAX SB-C18(4.6mm×250mm,5μm)色谱柱,流动相A为乙腈,B为水,梯度洗脱:0-40min(A:30%→60%),40-50min(A:60%→30%);检测波长203nm,流速1.0m L/min,柱温30℃。结果:重楼皂苷Ⅰ在0.0684-4.38μg,重楼皂苷Ⅱ在0.0675-4.32μg,重楼皂苷Ⅵ在0.0703-4.50μg,重楼皂苷Ⅶ在0.0691-4.42μg范围内线性关系良好,平均加样回收率分别为99.456%、100.593%、99.447%、99.812%;RSD(n=6)分别为1.771%、1.189%、1.682%、2.167%。结论:该方法精密度和重现性好,结果准确,可以用于癌痛平的质量控制。
OBJECTIVE: To establish a method for the simultaneous determination of the contents of Ⅰ, Ⅱ, Ⅵ and Ⅶ in Campanulaceae by RP-HPLC. Methods: Agilent ZORBAX SB-C18 column (4.6mm × 250mm, 5μm) was used. Mobile phase A was acetonitrile and B was water. The gradient elution was 0-40min (A: 30% → 60% A: 60% → 30%); detection wavelength of 203nm, flow rate of 1.0m L / min, column temperature 30 ℃. Results: The polysaccharide Ⅰ was 0.0684-4.38μg, polysaccharide Ⅱ was 0.0675-4.32μg, polysaccharide saponin Ⅵ was 0.0703-4.50μg, polysaccharide saponin Ⅶ was in the range of 0.0691-4.42μg, the average was The recoveries were 99.456%, 100.593%, 99.447% and 99.812%, respectively. The RSDs (n = 6) were 1.771%, 1.189%, 1.682% and 2.167%, respectively. Conclusion: The method has good precision and reproducibility with accurate results and can be used for quality control of cancer pain.