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作者用小鼠抗DNP的IgE抗体作为抗原,免疫大鼠,然后取其脾脏与小鼠骨髓瘤细胞SP2/0融合,筛选得到两个分泌抗小鼠IgE的单克隆抗体(McAb)的克隆——No.297、No。334。抗小鼠IgE的McAb的滴度用ELISA法测得的抗体最高稀释度的倒数来表示,活性用McAb抑制小鼠IgE被动皮肤过敏反应(PCA)和诱发的小鼠IgE致敏大鼠皮肤的反向被动转移皮肤反应(PK)表示。抗地鼠胸腺细胞球蛋白(ATG)是用10~8新鲜地鼠胸腺细
The mouse was immunized with the anti-DNP IgE antibody as an antigen, then the spleen of the mouse was fused with mouse myeloma cells SP2 / 0 to obtain two clones of monoclonal antibody (McAb) secreting anti-mouse IgE. -No.297, No. 334. The titer of the McAb against mouse IgE is expressed as the reciprocal of the highest dilution of the antibody measured by the ELISA, and the activity of the mouse skin is sensitized to the mouse by the McAb against the mouse IgE passive skin allergy (PCA) and the induced mouse IgE Reverse passive skin reaction (PK) expression. Anti-hamster thymocyte globulin (ATG) is used in 10 to 8 gilts of fresh gilts