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胶质芽胞杆菌WY120是微生物肥料生产常用菌株,传统的生物钾肥菌剂发酵生产的主要原料是采用淀粉、蔗糖和葡萄糖等。以玉米秸秆为原料,通过与淀粉蔗糖培养基发酵培养胶质芽胞杆菌的比较研究,探讨了玉米秸秆酶解液作为碳源发酵生产胶质芽胞杆菌的可行性。结果显示,玉米秸秆酶解液作为碳源发酵生产的胶质芽胞杆菌,其活菌数为3.52×108cfu/mL,是淀粉蔗糖培养基的1.63倍。秸秆酶解液培养基的优化试验结果显示,其最佳初始葡萄糖浓度为15 g/L,活菌数可达到3.95×108cfu/mL。当转速为250 r/min,pH控制在7.0～7.2时,5 L发酵罐的试验结果显示,其活菌数高达6.76×108cfu/mL,是摇瓶培养的1.71倍。研究结果表明,玉米秸秆可替代传统的淀粉蔗糖为碳源发酵生产胶质芽胞杆菌,为玉米秸秆资源化利用提供了一种新途径。
Gliocladium Bacillus WY120 is a common microbial fertilizer production strains, the traditional biological potassium fertilizer production of fermented raw materials is the use of starch, sucrose and glucose. The feasibility of using fermented corn stalk as raw material to produce gliadin from Bacillus subtilis by fermenting with Starch sucrose was studied. The results showed that corn stalk hydrolyzate was used as carbon source for the production of Bacillus gibberellis. The viable count was 3.52 × 108 cfu / mL, which was 1.63 times of that of starch sucrose medium. The optimization of straw enzymolysis medium showed that the optimal initial glucose concentration was 15 g / L and the viable count reached 3.95 × 108 cfu / mL. When the rotating speed was 250 r / min and the pH was controlled at 7.0-7.2, the test result of 5 L fermenter showed that the viable count was 6.76 × 108 cfu / mL, which was 1.71 times of shake flask culture. The results showed that corn stalks can replace the traditional starch sucrose as carbon source for the fermentation of gliadin, which provided a new way for the utilization of corn stalks.