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目的：建立一种新的用于肾移植术后人巨细胞病毒（ＨＣＭＶ）感染的快速定量诊断方法。方法：采用半巢式核酸荧光定量技术（ＡｍｐｌｉＳｅｎｓｏｒ－ＰＣＲ）检测已知含量的ＨＣＭＶ－ＡＤ１６９标准病毒株、无关病毒、无关细菌及临床血标本的ＨＣＭＶ－ＤＮＡ和质粒标准品，以确定该方法的敏感性、特异性和定量的准确性。结果：对已知含量的标准病毒株的定量检测结果与其已知含量基本一致；无关病毒包括单纯疱疹病毒１型（ＨＳＶ－１）、单纯疱疹病毒２型（ＨＳＶ－２）、ＥＢ病毒（ＥＢＶ）、丙型肝炎病毒（ＨＣＶ）及无关细菌包括金黄色葡萄球菌、结核杆菌等均为阴性；文中还检测肾移植受者２０例，结果半巢式核酸荧光定量技术的阳性率高于白细胞中的ＨＣＭＶ抗原（ＨＣＭＶ－Ａｇ）血症的阳性率；ＨＣＭＶ质粒标准品的最高检测敏感度为２５０拷贝／毫升；使用该方法标本处理简单，６小时内得出结果。结论：该方法快速，定量较准确，特异性、敏感性较高，是一种临床上值得推广的应用技术
Objective: To establish a new rapid quantitative diagnostic method for human cytomegalovirus (HCMV) infection after renal transplantation. Methods: HCMV-AD169 standard virus, HCMV-DNA and plasmid standards of unrelated bacteria and clinical blood samples were detected by semi-nested fluorescent quantitative assay (AmpliSensor-PCR) Sensitivity, specificity and quantitative accuracy. Results: The results of quantitative detection of the standard virus strains with known contents were basically consistent with their known contents. The unrelated viruses included HSV-1, HSV-2, EBV ), Hepatitis C virus (HCV) and unrelated bacteria including Staphylococcus aureus, Mycobacterium tuberculosis and so on were negative. Twenty renal transplant recipients were also detected in this study. The positive rate of semi-nested-PCR was higher than that in white blood cells Of HCMV antigen (HCMV-Ag) positive rate of sera; HCMV plasmid standard maximum detection sensitivity of 250 copies / ml; using the method of specimen processing is simple, the results within 6 hours. Conclusion: The method is rapid, accurate, specific and sensitive, and it is a kind of clinical technique worth popularizing