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利用免疫鸡的蛋黄制备了普通小麦高分子量谷蛋白1Dx5和1Bx17+1By18亚基的多克隆抗体,结果表明:鸡对抗原的免疫耐性较强,30～240μg抗原/只鸡都能产生较高效价的抗体,蛋黄中抗体效价略高于抗血清,两者的动态变化规律基本相同,但前者要晚7天左右.利用硫酸葡聚糖能有效地纯化蛋黄抗体.
The polyclonal antibodies of wheat HMW glutenin 1Dx5 and 1Bx17 + 1By18 subunits were prepared by using the egg yolk of immunized chicken. The results showed that the immune tolerance of the chicken to the antigen was strong, and the high titers of 30 ~ 240μg antigen / chicken Of the antibody, the antibody titer of egg yolk slightly higher than the antiserum, the dynamic changes of the two basically the same, but the former is about 7 days later. The use of dextran sulfate can effectively purify the yolk antibody.