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以老鸦瓣鳞茎芯芽和子茎(芽茎顶端膨大形成的子鳞茎)为外植体,探索噻苯隆(TDZ)和α-萘乙酸(NAA)诱导老鸦瓣外植体直接成芽的方法,建立老鸦瓣器官途径丛生芽组培体系。研究结果表明,芯芽和子茎在MS+TDZ 2.0 mg·L-1+NAA 2.0 mg·L-1和MS+TDZ 2.0 mg·L-1+NAA 4.0 mg·L-1培养基中可直接诱导不定芽,芽诱导率分别为72.92%,79.22%;利用子茎不定芽在MS+TDZ 0.2 mg·L-1+NAA 0.2 mg·L-1培养基中可进行丛生芽增殖,增殖系数2.23,形成的芽体健壮,叶色嫩绿;增殖后的丛生芽在含有IBA 1.0 mg·L-1的MS培养基中即可生根,生根率52.6%,平均3~5条根。利用老鸦瓣芯芽和子茎,筛选出了老鸦瓣芯芽和子茎直接诱导不定芽及丛生芽增殖和生根的最适培养基,建立了器官途径丛生芽组培体系。
In order to investigate the direct bud formation induced by TDZ and NAA in the bulbs of Bulbul petals and bulbs of bulbs, Establishing the organ pathway of carassius auratus tissue culture system. The results showed that the core buds and the stems could be directly induced in MS + TDZ 2.0 mg · L -1 + NAA 2.0 mg · L -1 and MS + TDZ 2.0 mg · L -1 + NAA 4.0 mg · L -1 The induction rate of adventitious buds and shoots was 72.92% and 79.22%, respectively. The adventitious buds of adventitious buds of MS + TDZ 0.2 mg · L-1 and NAA 0.2 mg · L- The buds formed were robust and the leaves were tender green. The multiplied buds could be rooted in MS medium containing 1.0 mg · L -1 of IBA, with the rooting rate of 52.6% and an average of 3-5 root. The optimal culture medium for the proliferation and rooting of adventitious buds and shoots of Populus pruinosa were screened out from the core shoots and the stems of Populus davidiana. The tissue culture system was established.