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目的研究荆芥内酯在大鼠胃肠道的吸收。方法应用大鼠在体胃肠吸收模型,采用酚红标记法校正肠循环液的体积,HPLC法测定不同时间点胃肠循环液中荆芥内酯的质量浓度。结果不同质量浓度(1.84、3.68、7.36μg/mL)的荆芥内酯在大鼠胃部每小时吸收率分别为(19.47±0.69)%、(21.66±1.92)%、(26.51±1.25)%;在大鼠肠道的吸收速率常数(Ka)分别为(0.203±0.007)、(0.159±0.011)、(0.134±0.012)h?1,各组之间差异均显著(P<0.05)。结论荆芥内酯在大鼠胃部的吸收可能为被动转运,在肠道的吸收可能为主动转运。
Objective To study the absorption of nepetalactone in rat gastrointestinal tract. Methods The rat gastrointestinal absorption model was applied. The volume of intestinal circulating fluid was corrected by phenol red labeling method. The concentration of nepetalactone in gastrointestinal fluid was determined by HPLC at different time points. Results The absorption rates of nepetalactone at different concentrations (1.84, 3.68, 3.36 μg / mL) in rat stomach per hour were (19.47 ± 0.69)%, (21.66 ± 1.92)%, (26.51 ± 1.25)% (0.203 ± 0.007), (0.159 ± 0.011) and (0.134 ± 0.012) h -1 in the intestine of rats, respectively. There was significant difference between the groups (P <0.05). Conclusion The absorption of nepetalactone in the stomach of the rats may be passive, and the absorption in the intestine may be the active transporter.