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为明确野生大豆的耐盐性及其在盐胁迫下的生理生态反应规律,用NaCl溶液模拟盐胁迫处理野生大豆幼苗,分析了不同胁迫程度和胁迫时间下相关指标的变化。结果表明:低浓度盐胁迫对其生长无显著影响,但随着胁迫浓度的增加和胁迫时间的延长,其鲜重的增加速率逐渐降低,植物组织含水率降低,根冠比增加,可溶性糖含量先大幅增加后稍有降低,可溶性蛋白含量先升高后降低,丙二醛(MDA)含量升高,游离脯氨酸(Pro)含量成倍增加。各指标的变化规律证明野生大豆具有一定的耐盐性,根冠比、含水率、可溶性糖、可溶性蛋白、MDA、Pro等是检测野生大豆抗盐性强弱的重要指标。
In order to clarify the salt tolerance of wild soybean and its physiological and ecological response under salt stress, the seedlings of wild soybean were treated with NaCl solution to simulate salt stress. The changes of related indexes under different stress degrees and stress time were analyzed. The results showed that low salt stress had no significant effect on its growth, but with the increasing of stress concentration and prolonging of stress time, the fresh weight gain rate gradually decreased, the water content of plant tissue decreased, the root / shoot ratio increased, soluble sugar content The content of soluble protein increased first and then decreased, the content of malondialdehyde (MDA) increased and the content of free proline (DMP) increased exponentially. The variation of each index proved that wild soybean had certain salt tolerance, root / shoot ratio, water content, soluble sugar, soluble protein, MDA and Pro were the important indexes to detect the salt resistance of wild soybean.