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目的研究泻心汤药中效成分黄芩苷、大黄酸、盐酸小檗碱在Caco-2细胞单层模型的吸收机制和相互作用。方法采用Caco-2细胞单层模型研究黄芩苷、大黄酸、盐酸小檗碱由绒毛面(AP侧)到基底面(BL侧)的转运过程,采用HPLC法测定药物在BL侧的累积量,通过计算表观渗透系数(Papp),比较配伍前后药物Papp的差别,探讨药物的跨细胞转运机制和相互作用。结果泻心汤药效成分黄芩苷、大黄酸、盐酸小檗碱在考察的浓度范围内皆以被动扩散为主要跨细胞转运方式,配伍前后药物的Papp无统计学差异。结论泻心汤中药效成分跨细胞转运方式为被动扩散,在转运过程中,药物之间无影响。
Objective To study the absorption mechanism and interaction of baicalin, rhein and berberine in Caco-2 cell monolayer model. Methods The monolayer model of Caco-2 cells was used to study the transport of baicalin, rhein, berberine from villus surface (AP side) to basal side (BL side) By calculating the apparent permeability coefficient (Papp), compare the difference between the drug Papp before and after compatibility, to explore the drug transcellular transport mechanism and interaction. Results XieXinTang active ingredients baicalin, rhein, berberine in the concentration range of investigation are passive diffusion as the main transcellular transport mode, before and after compatibility of the drug Papp no significant difference. Conclusion Xiexin Decoction effective component of transcellular transfusion method for passive diffusion, in transit, no effect between drugs.