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将硅烷偶联剂γ-缩水甘油醚氧丙基三甲氧基硅烷(KH560)键合到硅胶整体柱上,然后加入乙二胺与整体柱上的KH560的环氧基反应,形成柱体表面的氨基基团,再加入对甲苯磺酸,反应完毕制成芳基键合硅胶整体柱,并对其进行表征。利用该固定相,在0.1mol/LK2HPO4/KH2PO4缓冲盐(PBS)-硫酸铵体系下,对3种蛋白进行分离的结果表明,该芳基键合硅胶整体柱具有弱的疏水作用,在pH2～8范围内稳定性良好,柱压降较小,可有效用于蛋白质的快速分离。
The silane coupling agent γ-glycidoxypropyltrimethoxysilane (KH560) bonded to the silica gel monolithic column, and then added ethylenediamine monolithic column KH560 epoxy reaction to form the surface of the cylinder Amino groups, and then add p-toluenesulfonic acid, the reaction was completed to form an aryl bonded silica monolithic column, and its characterization. The separation of the three proteins in the 0.1 mol / L K2HPO4 / KH2PO4 buffer (PBS) -sulfate system showed that the monolithic silica column possessed a weak hydrophobic effect. Under the conditions of pH2 ~ 8 good stability within the range, the column pressure drop is small, can be effectively used for rapid protein separation.