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目的:构建油菜花粉HPLC指纹图谱,为油菜花粉质量评价以及质量控制提供依据。方法:以芦丁为内标物,采用Diamonsil C18柱(250mm×4.6mm,5μm)为分析色谱柱,乙腈-0.4%磷酸水溶液为流动相梯度洗脱,流速0.8mL/min。结果:10批不同产地油菜花粉的HPLC指纹图谱较相似,各成分得到较好地分离,并根据检测结果确定了10个共有指纹峰。精密度和重复性试验中,各共有指纹峰相对保留时间和相对峰面积的相对标准偏差(RSD)均在3%以内,符合指纹图谱技术要求。结论:该方法具有重现性好,特征性强,方法简便、快速等特点,能够客观反映油菜花粉的指纹特征,可以作为油菜花粉质量评价及控制的表征指标。
Objective: To construct HPLC fingerprinting of rape pollen and provide the basis for quality evaluation and quality control of rape pollen. Methods: Rutin was used as internal standard. Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) was used as the analytical column. The mobile phase of acetonitrile-0.4% phosphoric acid was eluted with a gradient of 0.8 mL / min. Results: The fingerprints of ten batches of pollen from different areas were similar and their components were separated well. According to the test results, 10 common fingerprint peaks were identified. In the precision and repeatability tests, the relative standard deviations (RSDs) of the relative fingerprint peaks and the relative peak areas of all the common fingerprint peaks were both within 3%, which accorded with the fingerprinting technique. CONCLUSION: The method is characterized by good reproducibility, strong characteristic, simple and rapid method and can objectively reflect the fingerprinting characteristics of rape pollen, which can be used as the indicator of quality evaluation and control of rape pollen.