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目的:探讨热药吴茱萸对虚寒证大鼠肝脏能量代谢的影响。方法:大鼠利血平皮下注射造模,经吴茱萸高剂量(4.2g生药/kg)、低剂量(2.1g生药/kg)灌胃给药干预12天后,测定血清NEAF含量、肝组织SDH、Na+-K+-ATP酶、Ca2+-ATP酶活性、肝脏组织中ATP的含量及EC值。结果:利血平虚寒证大鼠血清NEFA值下降,肝组织SDH活力、Na+-K+-ATPase、Ca2+-ATPase活力均显著降低,ADP、AMP及TAN含量均明显升高,EC值明显降低;吴茱萸高、低剂量均能极显著升高NEFA值,吴茱萸高剂量极显著升高SDH活力,吴茱萸低剂量有升高SDH活力的趋势,吴茱萸高、低剂量能明显降低ADP、AMP及TAN含量,升高EC值;吴茱萸高、低剂量组Na+-K+-ATPase活力显著下降。结论:热药吴茱萸可促进虚寒证大鼠ATP的生成,可能与吴茱萸提高NEFA含量及SDH活性,促进线粒体呼吸功能,提高线粒体氧化磷酸化效率有关。
Objective: To investigate the effect of Evodia rutaecarpa on energy metabolism in the liver of asthenic rats. Methods: Rats were injected subcutaneously with reserpine. After high dose of Evodia rutaecarpa (4.2g crude drug / kg) and low dose (2.1g crude drug / kg) intragastric administration for 12 days, the content of NEAF, the content of SDH, Na + -K + -ATPase, Ca2 + -ATPase activity, ATP content in liver tissue and EC value. Results: The serum NEFA level of reserpine deficiency syndrome rats decreased, the activities of SDH, Na + -K + -ATPase and Ca2 + -ATPase in liver tissues were significantly decreased, while the levels of ADP, AMP and TAN were significantly increased and the EC values were significantly decreased. Evodia, high and low doses of Evodia can significantly increase the NEFA value, Evodia high dose significantly increased SDH activity, Evodia low dose SDH activity trends, Evodia high and low doses can significantly reduce ADP, AMP and TAN content, Increasing the EC value; Evodia, high and low doses of Na + -K + -ATPase activity decreased significantly. CONCLUSION: Evodia rutaecui can promote the ATP production in rats with deficiency of cold, which may be related to the improvement of NEFA content and SDH activity of Evodia Rutaecarpa, promotion of mitochondrial respiratory function and enhancement of mitochondrial oxidative phosphorylation efficiency.