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目的检测非小细胞肺癌(NSCLC)组织中E1A因子(E1AF)、基质溶解素(MMP-7)、尿激酶纤溶酶原激活物(uPA)的表达及其与临床病理特征之间的关系。方法采用免疫组织化学技术检测56例NSCLC组织及其癌旁配对正常肺组织中MMP-7、uPA蛋白的表达,采用RT-PCR方法检测E1AFmRNA的表达。结果与癌旁配对正常肺组织相比,NSCLC中E1AFmRNA、MMP-7和uPA表达明显升高(P<0.01),E1AF表达与MMP-7及uPA的表达分别呈正相关(P<0.05);MMP-7与uPA表达无显著相关性(P>0.05);E1AF、MMP-7、uPA表达均与组织类型无关,E1AF、MMP-7与分化程度有关,uPA与肿瘤大小有关,三者均与TNM合并分期和淋巴结转移密切相关(P<0.05)。结论 E1AF、MMP-7和uPA在NSCLC组织中的表达与肿瘤的浸润、转移有关,E1AF可能在转录水平上调控MMP-7和uPA的表达,E1AF基因可作为肺癌基因诊断和治疗的新靶点,联合检测有助于判断患者的预后。
Objective To investigate the expression of E1A, MMP-7 and uPA in non-small cell lung cancer (NSCLC) and their relationship with clinicopathological features. Methods Immunohistochemical technique was used to detect the expression of MMP-7 and uPA in 56 cases of NSCLC tissues and paracancer normal lung tissues. The expression of E1AF mRNA was detected by RT-PCR. Results The expression of E1AF mRNA, MMP-7 and uPA in NSCLC was significantly higher than that in paracancer normal lung tissue (P <0.01). The expression of E1AF was positively correlated with the expression of MMP-7 and uPA (P <0.05) -7 had no significant correlation with uPA expression (P> 0.05). The expression of E1AF, MMP-7 and uPA had no correlation with the type of tissue. E1AF and MMP-7 were related to the degree of differentiation. Staging and lymph node metastasis were closely related (P <0.05). Conclusion The expression of E1AF, MMP-7 and uPA in NSCLC tissues is related to tumor invasion and metastasis. E1AF may regulate the expression of MMP-7 and uPA at the transcriptional level. E1AF gene may serve as a new target for lung cancer gene diagnosis and treatment , Joint testing can help determine the prognosis of patients.