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藻蓝蛋白亚基(PCS)是一种低毒并具良好荧光活性的光敏剂.为了增加PCS对乳腺癌细胞的PDT疗效,制备了聚乙二醇修饰的藻蓝蛋白亚基脂质体(PEG-PCS-lip).该脂质体的平均粒径为(136.3±7.6)nm,包封率达到52.1%.在浓度为100mg/L时,PEG-PCS-lip组在MCF-7细胞中的转染率在5h达到(29.1±1.2)%,是PCS-lip组的1.5倍,PCS组的3.1倍.PEG-PCS-lip光动力作用半致死剂量对MCF-7及MA-782细胞分别为(65.4±2.47)mg/L及(70.5±2.95)mg/L.用100mg/L的PEG-PCS-lip进行PDT处理后凋亡率达到了28.5%.与PCS相比,PEG-PCS-lip在血液和肿瘤中比在其他组织中聚集的浓度更高、时间更久,并在注射10h后达到峰值.PCS-PEG-lip-PDT处理组(每千克体重静脉给药10mg,照射剂量为150J/cm2)的肿瘤生长率下降至8.4%,而同时对照组肿瘤大小约增加了两倍.HE组织切片染色和透射电镜观察发现PEG-PCS-lip-PDT组的肿瘤细胞经历了凋亡和坏死.这些数据都表明,相比于PCS和PCS-lip,PEG脂质体能够增强PCS在肿瘤细胞聚集的靶向性,提高PDT疗效.
Phycocyanin subunit (PCS) is a kind of photosensitizer with low toxicity and good fluorescence activity.In order to increase the PDT effect of PCS on breast cancer cells, a modified polyethylene glycol-modified phycocyanin subunit liposome PEG-PCS-lip) .The liposomes had an average particle size of (136.3 ± 7.6) nm with an encapsulation efficiency of 52.1%. At the concentration of 100 mg / L, (29.1 ± 1.2)%, which was 1.5 times that of PCS-lip group and 3.1 times that of PCS group.PEG-PCS-lip photodynamic effect of semi-lethal dose on MCF-7 and MA-782 cells (65.4 ± 2.47) mg / L and (70.5 ± 2.95) mg / L. The apoptosis rate of PDT treated with 100 mg / L PEG-PCS-lip reached 28.5% Lip concentration in blood and tumor was higher than that in other tissues for a longer time and peaked 10h after injection.PCS-PEG-lip-PDT treatment group (10mg intravenously per kilogram of body weight, radiation dose The tumor growth rate of PEG-PCS-lip-PDT group was reduced to 8.4%, while the tumor size in the control group almost doubled at the same time.HE staining and transmission electron microscopy showed that the tumor cells in PEG-PCS-lip-PDT group experienced apoptosis and Necrosis These data show that compared to PCS and PCS-lip, PEG liposomes can enhance the PCS in the aggregation of tumor cells targeted to improve the efficacy of PDT.