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目的研究胃腺癌和癌旁组织中β-连环蛋白和c-myc的表达及其临床意义。方法收集102例胃腺癌患者的手术切除标本共297份,其中取自癌组织102份,癌旁组织195份(肿瘤边缘2cm以内97份,5cm以外98份)。将标本分别制成282点、156点和156点三块组织芯片,采用免疫组织化学法检测胃组织芯片中β-连环蛋白和c-myc的表达。结果β-连环蛋白和c-myc基因表达在胃黏膜癌变过程中呈上升趋势,从肠上皮化生开始即有明显增高,在胃癌显著高于肠上皮化生(P＜0．0 5,P＜0．001)。β-连环蛋白的异常表达与胃腺癌的分化程度及浸润深度有关(P＜O．05),c-myc表达与胃腺癌的分化程度有关(P＜0．05)。胃腺癌中β-连环蛋白和c-myc表达呈明显正相关(P＜0．05)。结论β-连环蛋白异常表达可能激活靶基因c-myc的表达,并在胃腺癌发生发展中起重要作用。
Objective To study the expression of β-catenin and c-myc in gastric adenocarcinoma and paracancerous tissues and its clinical significance. Methods Totally 297 surgical specimens of 102 patients with gastric adenocarcinoma were collected, including 102 cancer tissues and 195 adjacent tissues (97 lesions within 2 cm of the tumor and 98 lesions beyond 5 cm). The specimens were made into 282 pieces, 156 points and 156 points of three tissue microarrays. The expression of β-catenin and c-myc in gastric tissue was detected by immunohistochemistry. Results The expressions of β-catenin and c-myc were up-regulated during the carcinogenesis of gastric mucosa, which was significantly higher than that of intestinal metaplasia (P <0.0 5, P <0.001). The abnormal expression of β-catenin correlated with the differentiation degree and depth of invasion of gastric adenocarcinoma (P <0.05). The expression of c-myc was correlated with the differentiation of gastric adenocarcinoma (P <0.05). The expression of β-catenin and c-myc in gastric adenocarcinoma was positively correlated (P <0.05). Conclusion The abnormal expression of β-catenin may activate the expression of target gene c-myc and play an important role in the development of gastric adenocarcinoma.