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目的:探讨精子蛋白在成年男性弱精子症中的差异表达情况。方法:用iTRAQ标记以及二维高效液相色谱/串联质谱联用的方法研究成年男性正常精子以及轻、中、重度弱精症患者的精子蛋白表达情况。结果:以正常组作为参照,本研究共发现1 073个蛋白质与弱精症相关。其中,与正常相比,轻度、中度和重度精子表达上调蛋白数为474,232和311,精子表达下调蛋白数为529,800和719,上调和下调的蛋白质数量在弱精子症患者组构成比存在明显差别(P<0.05),以蛋白质表达数量降低为主。经蛋白功能注释,弱精症中明显上调和下调的蛋白质主要为结合、酶催化、分子结构、酶调节以及氧化还原功能等相关蛋白。其中,38个蛋白明确与精子发生、精子活动及受精过程相关。结论:采用iTRAQ标记技术能很好的应用于弱精症精子蛋白质组学研究。同时,弱精症中部分精子蛋白质表达以蛋白质数量降低为主,并且其变化幅度与弱精症严重程度相关。
Objective: To investigate the differential expression of sperm protein in adult male asthenospermia. Methods: The sperm protein expression in adult male normal sperm and in mild, moderate and severe asthenospermia patients was studied by iTRAQ labeling and two-dimensional high performance liquid chromatography / tandem mass spectrometry. Results: The normal group as a reference, the study found a total of 1 073 proteins associated with asthenopia. Among them, compared with the normal, mild, moderate and severe sperm upregulation of protein 474,232 and 311, the number of down-regulated sperm protein 529,800 and 719, the number of up-and down-regulated protein composition in patients with asthenospermia was significantly Difference (P <0.05), with the decrease of protein expression. The protein function annotations, significantly elevated in the asthenospermia and downregulation of proteins are mainly binding, enzyme catalysis, molecular structure, enzyme regulation and redox function and other related proteins. Of these, 38 proteins were clearly associated with spermatogenesis, sperm motility and fertilization. Conclusion: The iTRAQ labeling technique can be applied to proteomics study of asthenospermia. At the same time, part of the sperm protein expression in asthenopia is mainly reduced by the amount of protein, and its magnitude of change is related to the severity of asthenospermia.