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目的:建立放射性肺损伤小鼠动物模型,研究放射性肺损伤病变规律。方法:用不同剂量60Cog射线进行小鼠全胸照射,采用光镜组织病理学检查及图像分析仪形态定量分析肺组织病变。结果:肺脏病变初期呈现急性炎症,以后转为慢性炎症和纤维化。与对照组相比,20或30Gy照射组小鼠肺泡间隔厚度,肺间质面密度增加,肺泡腔面积减小u001fp<0.05 ～p<0.01u001f。结论:用20Gy或30Gy可以建立放射性肺损伤小鼠动物模型,随照射剂量增大,肺部病变加重,肺纤维化出现提早。
OBJECTIVE: To establish an animal model of radiation-induced lung injury in mice and study the pathological changes of radiation-induced lung injury. Methods: Whole-chest irradiation of mice was induced by different doses of 60Cog radiation. Lung tissue lesions were quantitatively analyzed by light microscopy and histopathology. Results: The initial stage of lung lesions showed acute inflammation and later became chronic inflammation and fibrosis. Compared with the control group, the thickness of the alveolar septum and the interstitial density of the lung in the 20 or 30Gy irradiation group increased, and the area of the alveolar space was reduced (p <0.05 ~ p <0.01). Conclusion: Animal models of radiation-induced lung injury in mice can be established with 20Gy or 30Gy. Pulmonary fibrosis is premature with the increase of irradiation dosage, pulmonary pathological changes.