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目的 观察缓慢间断颅内加压法巴马小型猪脑死亡模型建立过程中血压和心率的变化。方法 巴马小型猪16头,随机分为2组:对照组(C组) ,5头,仅行麻醉维持;脑死亡组(B组) ,11头,应用改进的缓慢间断颅内加压法建立脑死亡模型。监测实验过程中动物平均动脉压(MAP)和心率(HR)变化。结果 B组中10头(10头/11头)建立脑死亡模型,1头(1头/6头)在开始颅内加压后“心肺死亡”。与C组比较,B组颅内加压后MAP和HR变化显著:(1)MAP和HR随着间断颅内加压呈波浪式上升和下降,总体呈升高趋势;颅内加压过程中MAP (176.91±3 3 .2 9)mmHg(1mmHg =0 .13 3kPa)和HR (182 .71±2 5 .67)次/分,与颅内加压前MAP (117.60±13 .75 )mmHg和HR(94.5 0±9.2 0 )次/分比较,差异有统计学意义(P <0 .0 5 )。(2 )确认脑死亡后,MAP和HR缓慢下降;直到MAP≤60mmHg或收缩压≤90mmHg ,需依赖液体支持来维持血压的稳定。与C组比较,确认脑死亡3h后,B组MAP差异有统计学意义(P <0 .0 5 ) ;确认脑死亡6h后,B组HR差异无统计学意义(P >0 .0 5 )。结论 颅内加压法建立巴马小型猪脑死亡模型过程中血压和心率呈特征性变化
Objective To observe the changes of blood pressure and heart rate during the process of brain-dead model of Bama miniature pigs by intracerebral pressure. Methods 16 Bama miniature pigs were randomly divided into 2 groups: control group (group C), 5, only to maintain anesthesia; brain death group (group B), 11, using an improved slow intercranial pressure to establish Brain death model. Animals were monitored for changes in mean arterial pressure (MAP) and heart rate (HR) during the experiment. Results In group B, 10 (11 rats) and 10 rats (11 rats) developed brain death model. One patient (1 patient / 6 rats) died of cardiorespiratory failure after intracranial compression. Compared with group C, the changes of MAP and HR in group B after intracranial pressurization were significant: (1) The MAP and HR increased and decreased with the increase of intracranial pressure intermittently. The intracranial pressure MAP (176.91 ± 3.322.9) mmHg (1mmHg = 0.133kPa) and HR (182.71 ± 2.57.6), respectively, were significantly lower than those before MAP (117.60 ± 13.75) mmHg And HR (94.5 0 ± 9.2 0) times / min, the difference was statistically significant (P <0.05). (2) MAP and HR decrease slowly after confirming brain death. Until MAP≤60mmHg or systolic blood pressure≤90mmHg, fluid support should be used to maintain the stability of blood pressure. Compared with group C, after 3 hours of brain death, MAP in group B was significantly different (P <0.05). After 6 hours of brain death, there was no significant difference in HR between groups B (P> 0.05). Conclusion The intracranial pressure method has the characteristic changes of blood pressure and heart rate during the process of establishing the Bama miniature pig brain death model