目的:研究都匀楼梯草Elatostema duyunens乙酸乙酯部位的化学成分。方法:利用硅胶色谱及重结晶进行化学成分分离与纯化,根据化合物的理化性质和光谱数据鉴定其结构。结果:从都匀楼梯草全草中分离鉴定了6个化合物,分别为:山奈酚-4’,7-二甲基-3-O-葡萄糖苷(1)、槲皮素-7-O-β-D-葡萄糖苷(2)、山奈酚-3-O-β-D-半乳糖苷(3)、洋芹素-7-O-β-D-葡萄糖苷(4)、胡萝卜苷(5)、棕榈酸(6)。结论:化合物1~6为首次从该植物中分离得到。 Objective: To study the chemical composition of ethyl acetate in Elatostema duyunens. Methods: Silica gel chromatography and recrystallization were used to separate and purify the chemical constituents. Their structures were elucidated on the basis of physicochemical properties and spectral data of the compounds. Results: Six compounds were isolated and identified from the whole plant of Duyun stairs, and were identified as kaempferol-4 ’, 7-dimethyl-3-O-glucoside (1) β-D-glucoside (2), kaempferol-3-O-β-D-galactoside (3) ), Palmitic acid (6). Conclusion: Compounds 1 ~ 6 were isolated from this plant for the first time.