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以油菜内源基因PEP、抗除草剂基因(BAR、PAT)、筛选基因NPTII、常见启动子(CaMV35S、FMV35S)和终止子NOS为检测对象,通过研究不同引物终浓度的配比以及退火温度对转基因菜籽粕多重PCR检测的影响,建立了菜籽粕转基因成分7重PCR检测体系。结果表明,本研究所建立的检测体系能有效检测出菜籽粕以及其他作物(大豆、玉米、大米、棉花籽)中的转基因成分,检测过程简便、准确,值得推广应用。
In this study, the endogenous PEP, BAR, PAT, NPTII, common promoter (CaMV35S, FMV35S) and terminator NOS of rapeseed (Brassica napus L.) The results of multiplex PCR detection of transgenic rapeseed meal established a 7-repeat PCR detection system for transgenic components of rapeseed meal. The results showed that the detection system established in this study can effectively detect the genetically modified components in rapeseed meal and other crops (soybean, corn, rice and cottonseed). The detection process is simple and accurate and it is worth to be popularized and applied.