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目的调查蒙古族、汉族、达斡尔族人群的ACE基因I/N的多态性,分析这些民族的遗传特点。方法应用ACE基因内含子的16多态部位侧翼的前体进行PCR扩增。等位基因在琼脂胶上电泳,溴乙啶染色,紫外灯下观察。结果蒙古族人群的DD,DI和Ⅱ基因型的频率是0.51,0.26和0.23;汉族的是0.33,0.32和0.35;达斡尔族人群的是0.26,0.60和0.13。结论ACE基因I/N的多态性在蒙古族、汉族和达斡尔族三民族的分布是不同的。
Objective To investigate the polymorphism of ACE gene I / N in Mongol, Han and Daur populations and to analyze the genetic characteristics of these ethnic groups. Methods The precursor flanking 16 polymorphic regions of intron of ACE gene was amplified by PCR. The alleles were electrophoresed on agar, stained with ethidium bromide and observed under UV light. Results The frequency of DD, DI and II genotypes in Mongolian population was 0.51, 0.26 and 0.23, respectively, for Han population was 0.33, 0.32 and 0.35, for Daur people was 0.26, 0.60 and 0.13 respectively. Conclusion The distribution of ACE gene I / N polymorphism is different among Mongol, Han and Daur ethnic groups.