人脐静脉内皮细胞在体外长期传代培养,国内尚未见报道。我们参照文献方法,从牛脑中提取内皮细胞生长因子样物质,以纤维蛋白粘接素(Fibronectin)为基质,研究人脐静脉内皮细胞的长期传代培养,获得成功。 将一定量的新鲜牛脑制成匀浆,经低温 Human umbilical vein endothelial cells in vitro long-term subculture, has not been reported in China. We refer to the literature method to extract endothelial cell growth factor-like substance from bovine brain and to study the long-term subculture of human umbilical vein endothelial cells with Fibronectin as a substrate. A certain amount of fresh bovine brain homogenized, the low temperature