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目的分析IL-12及SCF对CD34+细胞分裂增殖及IL-12Rβ的表达。方法分离正常足月产新生儿脐带血与健康成年人外周血中的单个核细胞,采用流式细胞术(FACS)比较2组中CD34+细胞的频率,分析IL-12及SCF对CD34+细胞的作用。结果健康成年人外周血中CD34+细胞平均频率为0.13%,显著低于新生儿脐带血中CD34+细胞的平均频率1.53%(P<0.001)。进一步研究表明,SCF可以促进CD34+细胞表达CD25;IL-12及SCF可以显著促进CD34+细胞的分裂增殖以及IL-12Rβ1的表达。结论新生儿脐带血中CD34+细胞的频率明显高于成年人外周血中CD34+细胞的频率。IL-12及SCF可以促进CD34+细胞的分裂增殖以及IL-12Rβ1的表达。
Objective To analyze the effects of IL-12 and SCF on CD34 + cell proliferation and IL-12Rβ expression. Methods Mononuclear cells from normal full-term neonatal umbilical cord blood and healthy adults were isolated and the frequency of CD34 + cells in two groups was compared by flow cytometry (FACS) to analyze the effect of IL-12 and SCF on CD34 + cells . Results The average frequency of CD34 + cells in peripheral blood of healthy adults was 0.13%, which was significantly lower than the average frequency of CD34 + cells in neonates’ cord blood (1.53%, P <0.001). Further studies showed that SCF can promote the expression of CD25 in CD34 + cells. IL-12 and SCF can significantly promote the proliferation and the expression of IL-12Rβ1 in CD34 + cells. Conclusion The frequency of CD34 + cells in neonatal cord blood is significantly higher than that of CD34 + cells in adult peripheral blood. IL-12 and SCF can promote the division and proliferation of CD34 + cells and the expression of IL-12Rβ1.