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目的:探讨辛伐他汀对内皮细胞分泌内皮素-1(ET-1)的影响。方法:培养人脐静脉内皮细胞ECV304,分别用10,50,100μg/L不同浓度的肿瘤坏死因子-α(TNF-α)刺激,以50μg/LTNF-α刺激的基础上用辛伐他汀(0.1、1.0、10.0μmmol/L)及10μmol/L辛伐他汀+0.2mmol/L甲羟戊酸共育,用放射免疫方法测定ET-1的浓度。结果:不同浓度的TNF-α刺激24h后,培养上清ET-1浓度显著高于空白对照组(均P<0.01);与TNF-α(50μg/L)干预组相比,各浓度的辛伐他汀能明显减少ET-1生成,而该作用可被甲羟戊酸逆转。结论:辛伐他汀可以抑制TNF-α诱导的ET-1的生成,该作用可被甲羟戊酸逆转。
Objective: To investigate the effect of simvastatin on endothelin-1 (ET-1) secretion in endothelial cells. Methods: Human umbilical vein endothelial cells (ECV304) were cultured and stimulated with 10,50,100μg / L of TNF-α at a concentration of 0.1μg / L and 50μg / 1.0, 10.0 micromol / L) and 10 micromol / L simvastatin + 0.2 mmol / L mevalonic acid. The concentrations of ET-1 were determined by radioimmunoassay. Results: Compared with the control group, the concentration of ET-1 in the supernatant of the culture supernatant was significantly increased after the different concentrations of TNF-α were stimulated for 24 hours (all P <0.01). Compared with the intervention group of TNF-α (50μg / L) Valvastatin significantly reduced ET-1 production, which can be reversed by mevalonate. Conclusion: Simvastatin can inhibit TNF-α-induced ET-1 production, which can be reversed by mevalonate.