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目的探讨软骨细胞在体内非软骨形成部位促进骨髓基质细胞(BMSCs)向软骨分化并形成软骨的可行性。方法猪BMSCs与软骨细胞按一定比例(6∶4或7∶3)混匀,取2.5×107个混合细胞悬浮于0.5ml30%Pluronic溶液后注射到裸鼠皮下(n=6)。相同数量的单纯软骨细胞或BMSCs同样方法注射,分别作为阳性对照及阴性对照,0.75×107个软骨细胞同样注射作为低浓度软骨细胞对照。各组均8周后取材检测。结果混合细胞组及阳性对照组均形成了成熟的软骨。组织学可见成熟软骨陷窝、异染基质及Ⅱ型胶原表达。两组新生软骨糖胺多糖(GAG)含量差异无统计学意义(P>0.05),两混合组平均湿重分别为(320±48)mg和(294±37)mg,均达到阳性对照组70%以上。BMSCs组仅形成了纤维性组织,低浓度软骨细胞组在局部形成了少量软骨,但新生软骨平均湿重低于阳性对照的30%。结论上述结果提示软骨细胞能诱导BMSCs在体内非软骨形成部位向软骨分化并形成软骨组织。
Objective To investigate the feasibility of chondrocytes promoting the differentiation of bone marrow stromal cells (BMSCs) into cartilage and forming cartilage in the non-cartilage site in vivo. Methods Porcine BMSCs and chondrocytes were mixed in a certain ratio (6: 4 or 7: 3). 2.5 × 107 mixed cells were subcutaneously injected into 0.5 ml of 30% Pluronic solution and subcutaneously injected into nude mice (n = 6). The same number of simple chondrocytes or BMSCs were injected in the same manner as the positive control and the negative control, respectively, and 0.75 × 107 chondrocytes were also injected as low-concentration chondrocytes control. Each group were taken after 8 weeks of testing. Results The mixed cell group and the positive control group formed mature cartilage. Histology showed mature cartilage lacuna, heterotrophic matrix and type Ⅱ collagen expression. There was no significant difference in the content of GAG between the two groups (P> 0.05). The average wet weight of the two mixed groups were (320 ± 48) mg and (294 ± 37) mg respectively, reaching the positive control group 70 %the above. BMSCs only formed fibrous tissue. Low concentration of chondrocytes formed a small amount of local cartilage, but the average fresh weight of wet cartilage was lower than 30% of the positive control. Conclusion The above results suggest that chondrocytes can induce BMSCs to differentiate into cartilage and form cartilage tissue in the non-cartilage formation site in vivo.