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【目的】为了解不同枸杞产区枸杞根际真菌多样性的差异,从枸杞根际土壤真菌群落的角度试图解释宁夏枸杞优良品质与其土壤微生物菌群的联系。【方法】采用Mi Seq高通量测序方法,分别将我国4个不同地区枸杞根际土壤样品进行ITS区的高通量测序,并分析了物种组成和丰度、Alpha多样性、Beta多样性和菌群结构,测定不同产区枸杞主要有效成分,同时对枸杞品质和其土壤理化因子及枸杞根际真菌种群多样性的相关性做了分析。【结果】多糖含量和甜菜碱含量均是中宁枸杞高于兴仁和精河,格尔木含量最低;宁夏兴仁、宁夏中宁、新疆精河3个地区菌群结构类似,子囊菌门和结合菌门占总菌群的80%左右,而青海格尔木除了子囊菌门占58%外,壶菌门和新丽鞭毛菌门远远高于其他3个地区,比例占总菌群的近25%;4个地区枸杞根际土壤真菌菌群结构相似性依次为宁夏兴仁、新疆精河、宁夏中宁、青海格尔木。【结论】枸杞根际土壤样品真菌物种组成丰富,不同地区枸杞根际土壤真菌种群结构有一定差异;枸杞主要有效成分含量与枸杞根际土壤真菌种群结构具有一定相关性。
【Objective】 In order to understand the differences in the rhizosphere fungal diversity of Lycium barbarum in different wolfberry producing areas, attempts were made to explain the relationship between the fine quality of Lycium barbarum and the soil microbial flora in the rhizosphere soil fungal community of Lycium barbarum. 【Method】 The MiSeq high-throughput sequencing method was used to sequence high-throughput ITS region of Lycium barbarum rhizosphere soil in four different regions of China. The species composition and abundance, the diversity of Alpha, the diversity of Beta The results showed that the main effective components of Lycium barbarum in different regions were determined. The correlation between Lycium barbarum quality and soil physico-chemical factors and the diversity of Lycium barbarum fungi population was also analyzed. 【Result】 The content of polysaccharide and betaine in Zhongning wolfberry were higher than that in Xingren and Jinghe, and the content of Golmud was the lowest. The flora in Ningxia Xingren, Ningxia Zhongning and Jinghe, Xinjiang was similar in structure, Door accounted for about 80% of the total flora, and Golgi in Qinghai except Ascomycota accounted for 58%, Phyllanthus and Neisseria flammifer door is much higher than the other three regions, accounting for nearly 25% of the total flora; The similarity of the fungal community structure of rhizosphere soil in four areas was Xingren in Ningxia, Jinghe in Xinjiang, Zhongning in Ningxia and Golmud in Qinghai. 【Conclusion】 The results showed that the mycorrhizal species in the rhizosphere soil of the Lycium barbarum is rich in fungi, and the fungal population structure of the Lycium barbarum rhizosphere soil is different in different regions. The main active constituents of Lycium barbarum have some correlation with the fungal population structure of Lycium barbarum rhizosphere soil.