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目的 以树突状细胞 (DC)在体外诱导抗肝癌免疫。方法 自肝癌患者外周血中分离DC ;以粒 巨噬细胞集落刺激因子 (GM CSF)及白介素 4 (IL 4 )联合刺激DC ;以人肝癌细胞系HepG2细胞和BEL 74 0 2细胞的肿瘤相关抗原 (TAA)激活DC ;DC诱导自体T淋巴细胞增殖、分化为细胞毒素性T细胞 (CTL) ;检测CTL对HepG2细胞、BEL 74 0 2细胞、SGC 790 1细胞、LOVO细胞及HOS 86 0 3细胞的细胞毒作用。结果 肝癌患者外周血DC能够诱导自体T淋巴细胞增殖分化为CTL ,该CTL对HepG2细胞和BEL 74 0 2细胞有强大的杀伤力 (杀伤率分别为 90 %± 10 % ,86 %± 11% ) ,对SGC 790 1细胞、LOVO细胞及HOS 86 0 3细胞则无明显的细胞毒作用 (杀伤率分别为 11%± 6 % ,8%±4 % ,6 %± 4 % )。结论 肝癌患者外周血DC体外能够诱导高效而特异抗肝癌免疫。提示DC可能在治疗肝癌及预防肝癌术后复发和转移中发挥重要作用
Objective To induce anti-hepatocellular carcinoma immunity in vitro by dendritic cells (DC). Methods DCs were isolated from peripheral blood of patients with hepatocellular carcinoma; DCs were stimulated with granulocyte macrophage colony-stimulating factor (GM CSF) and interleukin 4 (IL 4); and tumor-associated antigens of human hepatocellular carcinoma cell lines HepG2 and BEL-7402 cells were obtained. (TAA) activates DC; DC induces proliferation of autologous T lymphocytes and differentiates into cytotoxic T cells (CTL); detection of CTLs on HepG2 cells, BEL7422 cells, SGC7901 cells, LOVO cells, and HOS 86030 cells The cytotoxic effect. Results Peripheral blood DCs in HCC patients were able to induce the proliferation and differentiation of autologous T lymphocytes into CTLs. The CTLs had a strong lethal effect on HepG2 cells and BEL-7402 cells (90 % ± 10 %, 86 % ± 11 % respectively). No significant cytotoxicity was observed in SGC7901 cells, LOVO cells, and HOS 86 0 3 cells (the kill rates were 11% ± 6%, 8% ± 4%, and 6% ± 4%, respectively). Conclusion Peripheral blood DCs from patients with hepatocellular carcinoma can induce efficient and specific anti-hepatocellular carcinoma immunity in vitro. DCs may play an important role in the treatment of liver cancer and prevention of recurrence and metastasis of liver cancer