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目的：探讨ＩＬ－８、ＴＮＦ－α在儿童支气管哮喘发病中的作用。方法：采用酶联免疫吸附试验（ＥＬＩＳＡ）检测３５例发作期、１８例稳定期哮喘患儿血清及外周血单个核细胞（ＰＢＭＣ）条件培养液中ＩＬ－８、ＴＮＦ－α水平。结果：哮喘发作期血清ＩＬ－８及ＴＮＦ－α明显增高，随病情缓解逐渐降低，达到或接近对照组水平。与对照组比较，哮喘发作期、稳定期ＰＢＭＣ条件培养液ＩＬ－８、ＴＮＦ－α水平显著增高；无论发作期抑或稳定期哮喘患儿ＰＢＭＣ经ＰＨＡ刺激所产生的ＴＮＦ－α、均显著高于未加ＰＨＡ刺激所产生的水平，而ＰＢＭＣ产生的ＩＬ－８则无类似变化。结论：儿童哮喘存在ＩＬ－８、ＴＮＦ－α合成或释放失调，二者可能参与哮喘气道慢性炎症的发生发展过程
Objective: To investigate the role of IL-8 and TNF-α in the pathogenesis of bronchial asthma in children. Methods: Serum and peripheral blood mononuclear cells (PBMCs) conditioned medium IL-8 and TNF-α levels were detected by enzyme linked immunosorbent assay (ELISA) in 35 cases of onset and 18 stable asthmatic children. Results: Serum levels of IL-8 and TNF-α increased significantly during the asthma attack and gradually decreased with the progression of the disease, reaching or approaching the level of the control group. Compared with the control group, the level of IL-8 and TNF-α in PBMC conditioned medium was significantly increased during the asthma attack and stable phase, and TNF-α produced by PHA stimulated PBMCs in both asthma and stable asthma children were significantly higher than those in control group There was no similar change in the level of IL-8 produced by PBMC without PHA stimulation. Conclusion: There is IL-8 and TNF-α synthesis or release disorder in childhood asthma, both of which may be involved in the development and progression of chronic airway inflammation in asthma