论文部分内容阅读
目的 研究乙型肝炎（ 乙肝） 病毒核心基因内缺失突变株（ Ｃ Ｉ Ｄ） 在慢性乙肝及肝癌患者中存在的状态及意义。方法 提取标本 Ｄ Ｎ Ａ 作 Ｓｏｕｔｈｅｒｎ 印迹及杂交，或用 Ｐ Ｃ Ｒ 扩增核心基因，分析片段大小，部分作克隆及测定。结果 ３５ 例慢性乙肝患者血清中３０ 例（８５ ％ ） 核心基因为阳性。３０例中３ 例（１０ ％ ） 除有正常大小片段外，还有相对小分子质量片段。１５ 例肝癌患者肝癌组织 Ｐ Ｃ Ｒ Ｃ 基因阳性为９ 例（６０ ％ ） ，未见小片段。肝癌组织中２ 份存在 Ｃ Ｉ Ｄ（２／９ ，２２ ％ ） 。克隆测序证明有大小不等的缺失突变，自６ ～３２４ 个核苷酸不等的符合框架的缺失，位于 Ｃ 基因的中段。结论 我国慢性乙肝患者中 Ｃ Ｉ Ｄ 株阳性率仅１０ ％ ，不是构成我国多数乙肝患者持续性病毒感染的主要原因。肝癌组织中 Ｃ Ｉ Ｄ 的阳性率高于乙肝患者血清的阳性率， Ｃ Ｉ Ｄ 在肝癌中的作用值得进一步研究。
Objective To study the status and significance of C I D deletion in hepatitis B virus (HBV) core gene in patients with chronic hepatitis B and liver cancer. METHODS: Southern blotting and hybridization were performed on samples of D N A, or the core genes were amplified by PCR. The size of the fragments was analyzed, and some were cloned and assayed. Results Thirty (85%) sera from 35 patients with chronic hepatitis B had positive core gene. Three of the 30 patients (10%) had relatively small molecular weight fragments in addition to normal size fragments. There were 9 cases (60%) of P C R C gene positive in HCC from 15 cases of hepatocellular carcinoma, no small fragment was found. Two of the liver cancer tissues had C I D (2/9, 22%). Cloning and sequencing showed that there were deletion mutations ranging in size from 6 to 324 nucleotides consistent with the lack of frame, located in the middle of the C gene. Conclusion The positive rate of C I D strain in chronic hepatitis B patients is only 10% in our country, which is not the main cause of persistent viral infection in most hepatitis B patients in our country. The positive rate of C I D in HCC was higher than that in patients with hepatitis B, and the role of C I D in HCC deserved further study.