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目的 :比较研究绿茶对铝钾矾、环磷酰胺 (Cyclophosphamide,简称 CP)诱发遗传物质损伤的修复作用。方法 :小白鼠骨髓细胞试验微核方法和小白鼠骨髓细胞染色体畸变试验方法。即以绿茶为阻断剂、以铝钾矾、环磷酰胺为诱变剂 ,测定小白鼠骨髓嗜多染红细胞 (PCE)的微核率和染色体畸变率。结果 :含 10 %绿茶的饲料使铝钾矾诱发的嗜多染红细胞微核率从 8.17± 1.6 0‰降至 3.6 7± 0 .82‰ (P <0 .0 0 1) ,染色体畸变率从 14.0 0± 2 .5 3降至 8.17± 1.47 (P <0 .0 0 1) ;含 10 %绿茶的饲料使环磷酰胺诱发的 PCE微核率从 32 .5 0± 3.5 1‰降至 19.33± 2 .5 8‰ (P<0 .0 0 1) ,染色体畸变率从 2 6 .83± 3.82降至 19.17± 2 .93 (P<0 .0 1)。结论 :表明绿茶对铝钾矾诱发微核、畸变的抑制率分别大于 CP诱发微核、畸变的抑制率 (P<0 .0 0 1) ,即铝钾矾诱发遗传物质损伤的抑制率大于环磷酰胺诱发遗传物质损伤的抑制率。
OBJECTIVE: To comparatively study the repairing effect of green tea on the damage of genetic material induced by alum sulphate and cyclophosphamide (CP). Methods: Micronucleus test of mouse marrow cells and chromosome aberration test of mouse marrow cells. In other words, micronucleus rate and chromosome aberration rate of mouse bone marrow polychromatic erythrocytes (PCE) were determined by using green tea as a blocker, aluminum alum and cyclophosphamide as a mutagen. RESULTS: The feed containing 10% green tea reduced the rate of micronuclei of pollens induced by polish from 8.17 ± 1.6 0 ‰ to 3.6 7 ± 0.82 ‰ (P <0.01), and the chromosome aberration rate decreased from 14.0 ± 2 .5 3 to 8.17 ± 1.47 (P <0. 001). The feed containing 10% green tea reduced the frequency of PCE induced by cyclophosphamide from 32.5 ± 3.5 1 ‰ To 19.33 ± 2.58 ‰ (P <0.01). The chromosome aberration rate decreased from 26.83 ± 3.82 19 to 19.17 ± 2.93 (P <0.01). Conclusion: The inhibitory rates of micronucleus and aberration induced by green tea are higher than that of micronucleus and aberration induced by CP (P <0.01), that is, the inhibition rate of genetic material damage induced by aluminum potassium sulfate is greater than that of ring Phosphoramide - induced inhibition of genetic material damage.