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Objective To investigate the mechanisms of delayed re-epitheliz at ion in wounds co mbined with whole-body irradiation(WBI )injury.Methods We o bserved re-epithelization in wo unds by histological methods and tra nsmission electron microscopy,detected the contents of proliferation cell nuclear antigen (PCNA)protein and mRNA using immunohistochemistry and in situ hybridization(I SH)methods.Results Re-epithelization in wounds combin ed with WBI injury was significantly delayed as compared with simple incision injury,and the contents of PCNA protein and mRNAsignificantly decreased too.Conclusions WBI injury has direct effect on wound epidermic cells,especially the suppress of epidermic ba sal cell proliferati on,which may be an important reason w hy wound combined wi th WBI injury hea ls more slowly.
Objective To investigate the mechanisms of delayed re-epitheliz at ion in wounds co mbined with whole-body irradiation (WBI) injury. Methods We o bserved re-epithelization in u unds by histological methods and tra nsmission electron microscopy, detected the contents of proliferation cell nuclear antigen (PCNA) protein and mRNA using immunohistochemistry and in situ hybridization (I SH) methods. Results Re-epithelization in wounds combin ed with WBI injury was significantly delayed as compared with simple incision injury, and contents of PCNA protein and mRNA signalsificly decreased too. Confcx WBI injury has direct effect on wound epidermic cells, especially the suppress of epidermic ba sal cell proliferati on, which may be an important reason w hy wound combined wi th WBI injury hea ls more slowly.