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测定了人红细胞摄入具有手性配体D-His和L-His(His=组氨酸)的金属配合物Ni~(2+)-D-His,Ni~(2+)-L-His,Cd~(2+)-D-His,Cd~(2+)-L-His的一级速率常数k,k_(Ni)~(2+)-D-His=2.0×10~(-3)h~(-1),k_(Ni)~(2+)-L-His=2.6×10~(-3)h~(-1),k_(Cd)~(2+)-D-His=k_(Cd)~(2+)-L-His=1.8×10~(-3)h~(-1),Ni配合物有手性选择性,Cd配合物则无手性选择性。这种手性选择性可能与配合物的几何构型有关。人红细胞对光学对映体配合物d-K[Co(EDTA)],1-K[Co(EDTA)],d-[Co(C_2O_4)(en)_2]I,1-[Co(C_2O_4)(en)_2)I,d-[Co(en)_3]I_3和1-[Co(en)_3)I_3(en=乙二胺)的摄入均显示了手性选择性。各对映体配合物对膜蛋白的巯基含量的影响无明显差别,但对双分子层脂膜(BLM)电位的影响却有非常显著的差别。这些事实说明,细胞摄入这类金属配合物的手性选择主要来源于对映体与膜脂的手性识别作用。
The metal complexes Ni ~ (2 +) - D-His, Ni ~ (2 +) - L-His with the chiral ligands D-His and L-His The first order rate constants k and k Ni2 + -D-His of Cd2 + -D-His and Cd2 + -L-His were 2.0 × 10-3 ) k -1 (2+) - L-His = 2.6 × 10 -3 h -1, k Cd 2+ + D-His = Cd (Cd 2+) - L-His = 1.8 × 10 -3 h -1, the chiral selectivity of the complexes was not obvious and the Cd complexes were not chiral. This chiral selectivity may be related to the geometrical configuration of the complex. The effect of human erythrocytes on the optical enantiomeric complexes dK [Co (EDTA)], 1-K [Co (EDTA)], d- [Co (C 2 O 4) ) _2) I, d- [Co (en) _3] I_3 and 1- [Co (en) _3) I_3 (en = ethylenediamine) all showed chiral selectivity. The enantiomeric complexes had no significant effect on the sulfhydryl content of the membrane proteins, but there was a very significant difference in the influence of the bilayer lipid membrane (BLM) potentials. These facts show that the chiral selection of the metal complexes by the cells mainly comes from the chiral recognition of enantiomers and membrane lipids.