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目的:研究Nestin、CK19、胰岛素、胰高血糖素及生长抑素在胚胎胰腺发育中的表达,探讨胰岛细胞分化发育的机制。方法:采用免疫组化SABC法及免疫组化双染法(LAB SA),对30例6～14wk人胚胎胰腺中,Nestin、CK19、胰岛素,胰高血糖素及生长抑素阳性的细胞进行定位。结果:(1)胚胎胰腺发育中Nestin阳性细胞存在于胰腺的间质,数量极少;CK19在胰腺导管上皮分化中持续表达,呈强阳性;(2)7wk胰腺导管上皮开始分化出胰岛细胞,胰岛素、胰高血糖素及生长抑素表达阳性,三者的表达并无时段差异性;随着胎龄的增加,阳性细胞数增加,14wk时胰岛逐渐形成,表达达到高峰。结论:胚胎胰腺的发育中,胰腺间质存在胰腺干细胞;胚胎早期胰腺导管上皮细胞开始分化并分泌胰岛素,其自分泌的激素可能参与调节胰岛细胞的迁移和胰岛的形成。
OBJECTIVE: To study the expression of Nestin, CK19, insulin, glucagon and somatostatin in embryonic pancreas and to explore the mechanism of islet cell differentiation and development. Methods: Immunohistochemical SABC method and immunohistochemical double staining (LAB SA) were used to locate Nestin, CK19, insulin, glucagon and somatostatin-positive cells in 30 6-14wk human embryonic pancreas . Results: (1) The number of Nestin positive cells in the pancreas during embryo development was extremely small. The expression of CK19 was strongly positive in pancreatic ductal epithelial cells. (2) The pancreatic ductal epithelium of 7wk began to differentiate into islet cells, The expressions of insulin, glucagon and somatostatin were positive. The expression of insulin, glucagon and somatostatin had no time difference. With the increase of gestational age, the number of positive cells increased and the islets formed gradually at 14wk. CONCLUSIONS: Pancreatic stem cells exist in the pancreas during the development of embryonic pancreas. Early embryonic pancreatic ductal epithelial cells begin to differentiate and secrete insulin. The autocrine hormones may be involved in the regulation of islet cell migration and islet formation.