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将氚标记的胸腺嘧啶核苷(3H-TdR)和尿嘧啶核苷(3H-UR)及~(32)P在体内标记胸腺中新合成的DNA与RNA,再用Schneider法将其分离,进行二苯胺和胎黑酚显色反应,分别测定DNA与RNA的含量,并根据同位素的掺入量来判断它们的合成速度。结果表明:在艾氏腹水癌(EAC)的生长早期,带瘤小鼠的胸腺中DNA与RNA的合成速度均明显加快,但DNA与RNA的含量却明显下降,用~(82)p追踪试验发现早期瘤鼠胸腺中~(32)P-DNA的排出速度比正常对照鼠快得多。
The tritiated thymidine (3H-TdR) and uridine (3H-UR) and ~(32)P were used to label newly synthesized DNA and RNA in the thymus in vivo and then separated by Schneider method. The color reaction between diphenylamine and cinnoquinol was used to determine the content of DNA and RNA, and their synthesis rate was judged based on the isotope incorporation. The results showed that in the early stage of growth of Ehrlich ascites carcinoma (EAC), the synthesis rate of DNA and RNA in the thymus of tumor-bearing mice was significantly accelerated, but the content of DNA and RNA was significantly decreased, using a ~(82)p follow-up test. It was found that the rate of 32P-DNA in the thymus of early tumor mice was much faster than that of normal control mice.