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β2肾上腺素受体(β2adrenergic receptor,β2AR)是G蛋白耦联受体(G protein coupled receptors,GPCRs)超家族中的一员,也是研究治疗哮喘的关键药物受体靶标.采用进化踪迹(evolutionary trace,ET)方法分析肾上腺素受体家族跨膜区片段序列,识别出了44个保守的残基,然后将β2肾上腺素受体以及受体D130N活性突变体、D79N失活突变体进行分子动力学模拟,试图找出与受体不同功能状态相关的结构动力学特征.发现受体DRY motif中的D130远离R131而转向K149残基这一结构特征与受体活性高度关联,此外,从残基相互作用的变化推断出了受体helix 2,4 and 6伴随着受体活化而发生的运动.这些研究结果对进一步探索β2肾上腺素受体突变体的激活机制以及所诱发疾病的分子机理提供了依据.
β2adrenergic receptor (β2AR), a member of the G protein coupled receptors (GPCRs) superfamily, is also a key drug receptor target for the study of asthma.It uses evolutionary trace , ET) analysis of the adrenaline receptor family transmembrane region fragment sequence, identified 44 conserved residues, and then β2 adrenergic receptor and receptor D130N activity mutant, D79N inactivation mutant molecular dynamics And try to find the structural dynamics related to the different functional status of the receptor.It is found that the structural feature of D130 in DRY motif away from R131 and turned to K149 is highly correlated with the activity of the receptor, The role of the changes deduced the movement of the receptors helix 2,4 and 6 with receptor activation.These results provide the basis for further exploration of the activation mechanism of β2 adrenergic receptor mutants and the molecular mechanism of the induced disease .