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目的:采用蛋白质组学方法对比分析高转移能力与低转移能力鼻咽癌细胞株,寻找差异表达蛋白质。方法:采用双向凝胶电泳技术分析高转移能力鼻咽癌细胞系S-18和低转移能力细胞系S-22,寻找S-18与S-22蛋白质表达差异。采用傅立叶变换离子回旋共振质谱技术,结合生物信息学数据库检索,鉴定出差异表达的蛋白质。结果:鉴定出12种差异表达蛋白质,包括转酮酶、NSFL1(p97)辅因子(p47)、磷酸肝油酸激酶1、醛缩酶A、核纤层蛋白A/C、膜联蛋白A2、SKP1G2等位基因抑制因子、不均一核核糖核蛋白C(C1/C2)、动力蛋白轻链Tctex型1、硫氧还蛋白、桩蛋白、丝氨酸/苏氨酸激酶受体关联蛋白。结论:这12种差异表达在鼻咽癌转移能力中可能起到关键性作用,为进一步研究鼻咽癌转移机制、分子标志及治疗靶点提供研究方向。
OBJECTIVE: To compare and analyze the nasopharyngeal carcinoma cell lines with high metastatic potential and low metastatic potential by proteomics to find differentially expressed proteins. Methods: Two-dimensional gel electrophoresis was used to analyze the high metastatic capacity of nasopharyngeal carcinoma cell line S-18 and low metastatic cell line S-22 in order to find the difference of S-18 and S-22 protein expression. Using Fourier transform ion cyclotron resonance mass spectrometry, combined with bioinformatics database search, identified differentially expressed proteins. RESULTS: Twelve differentially expressed proteins were identified, including transketolase, NSFL1 (p97) cofactor (p47), phospho-oleate kinase 1, aldolase A, lamin A / C, annexin A2, SKP1G2 Allele inhibitory factor, heterogeneous nuclear ribonucleoprotein C (C1 / C2), dynein light chain Tctex type 1, thioredoxin, paxillin, serine / threonine kinase receptor related proteins. CONCLUSION: These 12 differential expressions may play a key role in the metastasis of NPC, and provide the research direction for further studying the metastatic mechanism, molecular markers and therapeutic target of NPC.