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目的:研究水飞蓟宾对小鼠腹腔巨噬细胞释放纤维化因子的影响。方法:小鼠腹腔巨噬细胞先后用卡西霉素和脂多糖刺激培养24h诱导纤维化因子。巨噬细胞培养上清中促胶原合成活性和转化生长因子β活性分别采用3H-脯氨酸掺入法和雕肺上皮Mv-1-Lu细胞测定。结果:水飞蓟宾(6．25-50μg／mL)以浓度依赖方式抑制巨噬细胞产生胶原刺激活性和转化生长因子β1。结论:水飞蓟宾减少巨噬细胞释放促纤维化因子可能是其保肝抗硬化机制之一。
Objective: To study the effects of silybin on the release of fibrogenic factors from mouse peritoneal macrophages. METHODS: Mouse peritoneal macrophages were stimulated with kaximycin and lipopolysaccharide for 24 h to induce fibrogenic factors. The activity of collagen synthesis and the activity of transforming growth factor β in macrophages culture supernatants were measured by 3H-proline incorporation method and carvediosis lung epithelial Mv-1-Lu cells. RESULTS: Silybin (6.25-50 μg/mL) inhibited macrophage production of collagen-stimulating activity and transforming growth factor β1 in a concentration-dependent manner. Conclusion: Silibinin can reduce macrophages release pro-fibrogenic factors may be one of the mechanisms of hepatoprotective anti-hardening.